What is the role of phosphomolybdic acid?
What is the role of phosphomolybdic acid?
Phosphomolybdic is used as a stain for developing thin-layer chromatography plates, staining phenolics, hydrocarbon waxes, alkaloids, and steroids. Conjugated unsaturated compounds reduce PMA to molybdenum blue. Phosphomolybdic acid is also occasionally used in acid-catalyzed reactions in organic synthesis.
How does reticulin stain work?
Reticulin stains use silver and rely on the argyrophilic properties of the fibers. Argyrophilic cells can adsorb silver but cannot reduce it. “Adsorb” is a surface-based process, whereas “absorb” means there is permeation through the tissue.
What are special stains used for?
A special stain is a type of test that helps pathologists see details in a tissue sample that cannot be seen with the more common hematoxylin and eosin stain. Pathologists perform this type of test by adding a coloured dye (the stain) to a tissue sample which is then examined under a microscope.
Which stain is used for connective tissue?
Mason’s Trichrome stain is used to highlight connective tissue fibers in a tissue section. Collagen fibers are stained a blue hue, nuclei a blue-black, and cytoplasm, keratin, and muscle fibers a range of pink to red hues.
What is the function of phosphotungstic acid?
Phosphotungstic acid is used in histology as a component for staining of cell specimens, often together with haematoxylin as PTAH. It binds to fibrin, collagen, and fibres of connective tissues, and replaces the anions of dyes from these materials, selectively decoloring them.
What is the purpose of the phosphomolybdic acid PMA stain?
Stains for TLC Plates
| Name | Visualization of… | Comments |
|---|---|---|
| Phosphomolybdic Acid (PMA) | Universal stain Very effective against dilute sample | Visualization Colors Spots: Dark green to black BG: Light green |
| N.B.: Can be used for detection of alcohols, amines, sulfides and mercaptans groups when gently heated. |
What tissue components does Van Gieson’s stain?
Van Gieson’s stain is a mixture of picric acid and acid fuchsin. It is the simplest method of differential staining of collagen and other connective tissue. It was introduced to histology by American neuropsychiatrist and pathologist Ira Van Gieson.
What is differential staining technique?
Differential Staining is a staining process which uses more than one chemical stain. Using multiple stains can better differentiate between different microorganisms or structures/cellular components of a single organism.
What stains staining techniques are used to demonstrate the fungi?
The Grocott’s silver (GMS) stain is probably the most widely used fungal stain, however, the somewhat capricious nature of silver staining may challenge even the most experienced of histology practitioners.
Can Phosphotungstic precipitate proteins?
Many proteins derived from the latex of Hevea brasiliensis that remain soluble in trichloroacetic acid (TCA) can be precipitated by phosphotungstic acid (PTA). A combination of 5% TCA and 0.2% PTA precipitates a wide range of proteins effectively even when they are present in low concentrations (below 1 μg ml−1).
What functional groups does phosphomolybdic acid react with?
Visualization Summary
| UV Light: For aromatics + conjugated systems | Iodine: Visualizes ~half the time. Strongly reacts with aromatics |
|---|---|
| Permanganate: For alkenes, alkynes, or oxidizable groups (aldehydes, alcohols) | Phosphomolybdic Acid (PMA): For alcohols, phenols, alkenes, and many carbonyl compounds |